Find
Press Ctrl+F, or click Find in the edit bar, to open the find bar above the views. What you type decides what is searched:
- Three or more IUPAC letters search the sequence on both strands. Ambiguity codes work in the query:
GRCGYCfinds every BsaHI site, andNmatches any base. AnNin the sequence only matches anNin the query. Matches wrap around the origin of a circular sequence.Uis read asT, so an RNA is searched with the letters it is drawn with. - Anything else searches feature names and types, case-insensitively, so
tetfinds _tet_ and _tetR_, andpromoterfinds every promoter.
The counter shows which match is current, how many there are, and whether a sequence match is on the forward or reverse strand. The current match is selected and both views scroll to it, so anything that works on a selection (Add feature, Copy, Translate, Design primers) works on a find result.
While the bar is open, every match is drawn in both views as a dashed preview (see Previews), so you can see how the matches are spread over the molecule and not only where the next one is. The current one stays highlighted as the selection on top of that. Past 200 matches the views are left clear — the count answers the question better than 200 marks would. Nothing is added to the document, and the marks go when the bar closes.
Enteror ↓ goes to the next match,Shift+Enteror ↑ to the previous one.Escapeor Close closes the bar and leaves the last match selected.
How to find a restriction site by sequence
- Press
Ctrl+F. - Type the recognition sequence, for example
GAATTC. - Step through the hits with
Enter.
For a list of sites per enzyme, with cut positions and fragment sizes, use the Enzymes tab instead.