LOCUS       GW-pDEST             1837 bp    DNA     circular SYN 28-OCT-2026
DEFINITION  GW-pDEST, destination vector for the Gateway tutorial: promoter,
            attR1, ccdB and CmR stand-ins, attR2, terminator, AmpR and ori
            (synthetic example, invented sequence and invented att sites)
ACCESSION   .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        41..110
                     /label="synthetic promoter"
     protein_bind    123..162
                     /label="attR1"
                     /note="invented synthetic att site: arms around a 15 base core"
     CDS             173..472
                     /label="ccdB"
                     /note="synthetic stand-in for the ccdB gene"
     CDS             481..780
                     /label="CmR"
                     /note="synthetic stand-in for a resistance gene"
     protein_bind    791..830
                     /label="attR2"
                     /note="invented synthetic att site: arms around a 15 base core"
     terminator      843..897
                     /label="synthetic terminator"
     CDS             complement(948..1367)
                     /label="AmpR"
                     /note="synthetic stand-in for a resistance gene"
     rep_origin      1408..1807
                     /label="ori"
                     /note="synthetic stand-in for an origin"
ORIGIN
        1 tatcccatag gtagcatttt cgctggggaa aggtagacaa tatccgacta aaccgcaatg
       61 actgtaaagc agcgtaagac gacaacgtga gaatccggcc tattctagtg atccttcgtt
      121 ctacttctca gatcctgaga attgacctag tcagtacgtc aagagggatc gcatgtataa
      181 agacattagg gctagtgaga aaagggaacg gatccggcct acgctcttga tccgtgtcct
      241 aacaagcagg aaccaatcgt ttacaggtgt caccctctat ggcttttatc ggcgatcaca
      301 tgtaacacct atagacagcc cctatcaaaa gttttcgatt agcggagtcg tacaaaagca
      361 ccgcgctgta ctacgacggc gaactacgcc taccctcccc gctaatcact cacgctatct
      421 actagaggag ggtagacctc gtgccgtcat gccctatcaa aagggtacgt aacttctatt
      481 atgcgggtgg cgccagcccg tgctacctgc agaggaacga tcggagtacg tccaccgaca
      541 aaagcacggt cgcatgatgg tcagttaaca ctcaatgccg ggatgcacgg cctacgctat
      601 aaagtcagtt tctctccacg agtatcgtgc gaaatggtct ggtcgcccca aggacctatc
      661 ataagatcaa acattgcttg ttcggccttc tccttgcgag ctggctggca ccattttctg
      721 aaacgcaagg gcacggcttt gtcacattta agtatactat ctggggcttt aacagattaa
      781 gtctcgccca gtcaactgca tcgattgaca aggcttacga tctgactaag agaaatgaga
      841 gcgggagtag tggagtgggg atagtatatc cgcagagaca acgcgctttt tgctccagta
      901 accgccaaac attgcgagac cgttcactcc tacctgcaaa gaccttttta cgtcgcgaac
      961 agcagccacc cgcaattgaa cacgagaacc ttagcttctt ccgttatcga tctacagcct
     1021 gcacgaggta ggaaaacatt ggccaattgg cgtggatcac tccctacgaa aatgggtatt
     1081 atgttactaa tgtctcttct gtgatgatta gctttcgggt cgaggcgaac cccaccaacc
     1141 tggactgtct gacggtcaga ccaggtagat agacgcgggg cgaccggaca aactagaacg
     1201 atcataacca aggcacgtcc cgaaccttta atgccagtat ttaagtccat agtacataga
     1261 cggcccatgc aaagaccaag cacaccattt ccgtgcgagg gaacggccca acagtgggtg
     1321 agtggctctc gtgttttctt gggttcgctc tgcatgcaaa tcgccatatt gaaactaatg
     1381 caggcccaac ccccaaccct ctccagtggt agaccttcga tccatgacaa attccataag
     1441 ggtgatcgtt caacccctag gttgcttgga gaatcagcgg cacctacagc accagcaatt
     1501 cgaagaggtc ttagaacggc ggtcttagag agttacaccg catttacgtt tgtaccacgg
     1561 agaacgagtt tgtcactatg agtcttccca ttaagctagt cgggatacca taggctgtaa
     1621 caggcaaacg cccgagtgcc gtttcatgtc gtggtcagat ccatttggag tacgggtatc
     1681 tctcgggata gcgttatatc cgaatatact ttgaaccaac aagccccggt acgcaaggcc
     1741 gggagagagt gacgcagatg gcaggacaac aatgtgtaaa taggtggcaa aagtgtgttc
     1801 cccttcacat acaattctcg caataattcg gcattaa
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