LOCUS       pGG-Destination         2647 bp    DNA     circular SYN 28-OCT-2026
DEFINITION  pGG-Destination, synthetic example Golden Gate destination vector
            with a lacZ-dropout between two BsaI sites (synthetic example,
            invented sequence)
ACCESSION   .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             complement(65..877)
                     /label="KanR"
                     /undefined="synthetic stand-in for a resistance gene"
     rep_origin      978..1566
                     /label="ori"
                     /undefined="synthetic stand-in for an origin"
     misc_feature    complement(2331..2337)
                     /label="BsaI site"
     misc_feature    2338..2640
                     /label="lacZ-dropout"
                     /undefined="synthetic stand-in for a lacZ colour-marker
                     dropout"
     misc_feature    2641..2647
                     /label="BsaI site"
ORIGIN
        1 CGCTCGCATC GCCCGAGTGG AAGTTAATTA TGTGTTTCCA GCACGCGAGC ATGGTGAGAA
       61 TCGATTAAAT TCGCAGCCCA TGCCTACGGC CCAGTGCGTT AGTACACTTA CGAGTCGCAA
      121 TGCCGTATTT TCCTTGTTTC AACCCCGACG CTCCCGATAC TCCCATCAAG TAGTAATCTT
      181 CATTAGAGGC CATCATACAA CGAAATCGAC CGAAGATGCG CCTGCTATTG TAATTGGCAA
      241 CCTGAGCTGA TTCGATAAGT CTCCGACTCC GACCTCGAGT CGTCACAGTC TCAATAACGC
      301 GGTGCTGGAC AACAGGCTCG TGAAGAGAGT ATTCCATCTC AGGAAATCGA GAACCCGCGT
      361 TCACTAGCAT TCTGGAAAGG CGGATTGCGT GCAAGCAAAA CTGTCCTTCT GTAGGATGTC
      421 CTAGATAGAA TATAGGTGGG TGGTTAGTGC AGCATATACA GGGGAAAGTT AGTCCATTCT
      481 GCACGGGGAC GAGGAATGTT GACGGATGAG CCGGAGTGGC GGTTGTCTCA GGGCTATCCA
      541 GCAACTCTTC GACCCGTATA TAGACTGGTA GGGTAGAGGG CCATGCAACA ATAACCGAGC
      601 GGTGCTTGAC GTCACGTTTG TTACACAAGG CGCGGCGTTT TCTGGCGCCC GGCACCAACC
      661 GGGAGATGCA ACTCCGTTCA GCCGATAGCT CAACTGTACG CCAGAACGCG CTATGCCATA
      721 CACGGGCATT AGAAGGGCAA GCAATAGTCC GTGATGCGAG TCCAACGTGG AACAACACAG
      781 GTGAAGGTGT TTTCGCCCTT ACGTTCTTCC TCACAGTGGA AATTAGCAGT GACTTGGCAT
      841 TCCGCATACA CCCGGACGAT TTAAAAGCAG TTTCCATTAT AACGAACACT TGGATAGTGA
      901 GAGAGAGCCG CAAGGAGTCA GTTCATCTCA AATTGTGGGA CAGAGAGGAG GTTGACGGTC
      961 GTCGCCACCG GGCATTATTA CGCAAGTGCC TCTTTGGACC ACTTCGCGAG AGTATGTACT
     1021 AAGTTATTTT GTTCCGGTCC TATAAGCAGC TTTAACCGGT ATCCACTGGC TCTGTGAGCA
     1081 GTCGTCATAG CCTCTCTATT AGGTAACGAC TTTCGTGCGA AATTTCGCAA CGGATGTTTG
     1141 ATAGGCGAGG GGCTCTACTT CGAGTACTGC TGTGGAGCCA ACCGACCCAA CGCAGGCCAT
     1201 CCTTCGAGAT AAAACTACAA ATCACCGTCA CTCCCTTAAG TGAGTACCCT TAACCATATT
     1261 CCCCGCGGAT GAGTGGAGGA AGCCCTTCCA GAAGGTAGTT GTCTACTGAG ACAGCCCTGT
     1321 TCCTTAGTCG CAGAAACCAT AGAGTGCATT TTGACGCGCC ATCCCTGCGT CTACGCACCC
     1381 TTCTGGAGTA TTATTAGCTC AAGGCAGAGT CGGCGCCGCT GTGTTATGTT AGACTGCGGA
     1441 ATCTTCCCCT TTCTCTCCCT TCACGGGGCG CCACGCGCTG CGCGTAGTTG ACAAATTTAC
     1501 GTCTAGGGAA AGACTCTCCA AGTACAAATA TATAGTAAAC GACGCCCTCT GGGTTGGATA
     1561 TGCGTGGCAC AGTACGACCA TTGCCATCCA ACTGTAGCTA ATAAATGGCG AAACAGGAAC
     1621 GGTATTCTAT GAGGACCTTA CCAAGACAAT CAGATATACT TCACTTGCGT TCATGTAAGG
     1681 CGTGAGCCGT TATATGTAAC AAATCTACAC CCTTGCCTAC GAAGAAGCCC GACCACTACG
     1741 GAGCGATATA TAATGCTGTA CCCACAGGGC TGTCGTATGT CCCATTCACC TTCTTCGGGA
     1801 CTCAGCGGTC GGGTCTAACC TGTAGGTGGT TGTTATAGTC GCGAGTCGTA ACTCAGCCAA
     1861 GGGAAAACAA TGATTAATAG GCTGTGAGGA AGAGGAGATG AAACATTAAA TTACTTTGAG
     1921 TGGGACAGTT ACTCCCAAAT ACTGTTCGGT CAAATGTGTC TGATTACATG CCCATATAGC
     1981 AAATCTCGAG CCATATCGAT AGCTGTACTG AAGAAAACCA AACCCGTTTA GACGAAATCA
     2041 TCCTGAATAG CTACATACGC AAGCTGCATC ACGCAAGCCT AATCACAATA AGGTATTGCG
     2101 AGCGAAACGC GGTTTGAACT CGCATAACAC AAGGTATCTT TTGAAATCCA CTTCCCACAA
     2161 GGCACCTGTG GAGCGAGACT CGTAACGAGC TTTTGGAAAA GTTAATCACC CTTCACCAGA
     2221 ATCATCACTT CGACGCTGAC GCTCCCCAAG GACGGGACCC CAGCGAAGAA CTTATCGTGT
     2281 ACCGGGTAGG GTGATAAGCC TTTAACAATT CTACTCAACC TTTCGGGGAG AGAGACCATG
     2341 CGAGAGGCAG ATTTAGCCTG CCTCCGCCCG CTTAAGGGAA CTACTTTACC TACCGAGACA
     2401 GCGGGTCACA CAGGTGCTCA ACATCCGCTA CGCCGCTTGT GTCCTCCCAT GCCGTTATGG
     2461 AGGGCTGGAG TGGTACGTCT ACTGCAAAGG ACTGCGGCGG AGAAGAACGC GTGGTATCGG
     2521 ATGAGCACCC CGGCCGTACA CTTTTCCGGA GTCAAGATAA CTCTGAAAAA CTGGTGCTTA
     2581 GGAACGCCGG GTAGAAGCGG CCTTCCAAAA TATTTGTGGA CAGGCATACG CACTCGATAA
     2641 GGTCTCA
//