LOCUS       pGG-Expression          2857 bp    DNA     circular SYN 28-OCT-2026
DEFINITION  pGG-Expression, synthetic example Golden Gate expression vector: a
            promoter and a terminator with a lacZ-dropout between two BsaI
            sites, for a CDS part AATG-GCTT (synthetic example, invented
            sequence)
ACCESSION   .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             complement(61..873)
                     /label="KanR"
                     /undefined="synthetic stand-in for a resistance gene"
     rep_origin      974..1562
                     /label="ori"
                     /undefined="synthetic stand-in for an origin"
     promoter        2323..2442
                     /label="synthetic promoter"
     misc_feature    complement(2448..2453)
                     /label="BsaI site"
     misc_feature    2454..2756
                     /label="lacZ-dropout"
                     /undefined="synthetic stand-in for a lacZ colour-marker dropout"
     misc_feature    2757..2762
                     /label="BsaI site"
     terminator      2768..2857
                     /label="synthetic terminator"
ORIGIN
        1 cgcatcgccc gagtggaagt taattatgtg tttccagcac gcgagcatgg tgagaatcga
       61 ttaaattcgc agcccatgcc tacggcccag tgcgttagta cacttacgag tcgcaatgcc
      121 gtattttcct tgtttcaacc ccgacgctcc cgatactccc atcaagtagt aatcttcatt
      181 agaggccatc atacaacgaa atcgaccgaa gatgcgcctg ctattgtaat tggcaacctg
      241 agctgattcg ataagtctcc gactccgacc tcgagtcgtc acagtctcaa taacgcggtg
      301 ctggacaaca ggctcgtgaa gagagtattc catctcagga aatcgagaac ccgcgttcac
      361 tagcattctg gaaaggcgga ttgcgtgcaa gcaaaactgt ccttctgtag gatgtcctag
      421 atagaatata ggtgggtggt tagtgcagca tatacagggg aaagttagtc cattctgcac
      481 ggggacgagg aatgttgacg gatgagccgg agtggcggtt gtctcagggc tatccagcaa
      541 ctcttcgacc cgtatataga ctggtagggt agagggccat gcaacaataa ccgagcggtg
      601 cttgacgtca cgtttgttac acaaggcgcg gcgttttctg gcgcccggca ccaaccggga
      661 gatgcaactc cgttcagccg atagctcaac tgtacgccag aacgcgctat gccatacacg
      721 ggcattagaa gggcaagcaa tagtccgtga tgcgagtcca acgtggaaca acacaggtga
      781 aggtgttttc gcccttacgt tcttcctcac agtggaaatt agcagtgact tggcattccg
      841 catacacccg gacgatttaa aagcagtttc cattataacg aacacttgga tagtgagaga
      901 gagccgcaag gagtcagttc atctcaaatt gtgggacaga gaggaggttg acggtcgtcg
      961 ccaccgggca ttattacgca agtgcctctt tggaccactt cgcgagagta tgtactaagt
     1021 tattttgttc cggtcctata agcagcttta accggtatcc actggctctg tgagcagtcg
     1081 tcatagcctc tctattaggt aacgactttc gtgcgaaatt tcgcaacgga tgtttgatag
     1141 gcgaggggct ctacttcgag tactgctgtg gagccaaccg acccaacgca ggccatcctt
     1201 cgagataaaa ctacaaatca ccgtcactcc cttaagtgag tacccttaac catattcccc
     1261 gcggatgagt ggaggaagcc cttccagaag gtagttgtct actgagacag ccctgttcct
     1321 tagtcgcaga aaccatagag tgcattttga cgcgccatcc ctgcgtctac gcacccttct
     1381 ggagtattat tagctcaagg cagagtcggc gccgctgtgt tatgttagac tgcggaatct
     1441 tcccctttct ctcccttcac ggggcgccac gcgctgcgcg tagttgacaa atttacgtct
     1501 agggaaagac tctccaagta caaatatata gtaaacgacg ccctctgggt tggatatgcg
     1561 tggcacagta cgaccattgc catccaactg tagctaataa atggcgaaac aggaacggta
     1621 ttctatgagg accttaccaa gacaatcaga tatacttcac ttgcgttcat gtaaggcgtg
     1681 agccgttata tgtaacaaat ctacaccctt gcctacgaag aagcccgacc actacggagc
     1741 gatatataat gctgtaccca cagggctgtc gtatgtccca ttcaccttct tcgggactca
     1801 gcggtcgggt ctaacctgta ggtggttgtt atagtcgcga gtcgtaactc agccaaggga
     1861 aaacaatgat taataggctg tgaggaagag gagatgaaac attaaattac tttgagtggg
     1921 acagttactc ccaaatactg ttcggtcaaa tgtgtctgat tacatgccca tatagcaaat
     1981 ctcgagccat atcgatagct gtactgaaga aaaccaaacc cgtttagacg aaatcatcct
     2041 gaatagctac atacgcaagc tgcatcacgc aagcctaatc acaataaggt attgcgagcg
     2101 aaacgcggtt tgaactcgca taacacaagg tatcttttga aatccacttc ccacaaggca
     2161 cctgtggagc gagactcgta acgagctttt ggaaaagtta atcacccttc accagaatca
     2221 tcacttcgac gctgacgctc cccaaggacg ggaccccagc gaagaactta tcgtgtaccg
     2281 ggtagggtga taagccttta acaattctac tcaacctttc gggcggcaaa acgtccagac
     2341 tctccgcagt gacttatatg tcgtcccgtc ttccataagc agtagccgga agtgacaaaa
     2401 ttgcttatcc cagcggccgg tgttatgttt acccataccg taaatgagag accatgcgag
     2461 aggcagattt agcctgcctc cgcccgctta agggaactac tttacctacc gagacagcgg
     2521 gtcacacagg tgctcaacat ccgctacgcc gcttgtgtcc tcccatgccg ttatggaggg
     2581 ctggagtggt acgtctactg caaaggactg cggcggagaa gaacgcgtgg tatcggatga
     2641 gcaccccggc cgtacacttt tccggagtca agataactct gaaaaactgg tgcttaggaa
     2701 cgccgggtag aagcggcctt ccaaaatatt tgtggacagg catacgcact cgataaggtc
     2761 tcagcttagt gtcatcattg gggaaagtga ttcatgttgc ggcaggtact gcctctttct
     2821 acagtgctca atcatttagt attttttgct tagtgga
//